Description:Mouse monoclonal antibody to human cluster of differentiation 3 (CD3) antigen

Purification: Prootein G affinity purified

Product Type:Primary antibody

Target Protein:Human peripheral T cell CD3 antigen

Immunogen:Human peripheral T lymphocytes

Fusion Myeloma:Sp2/0-Ag14

Specificity:This antibody recognizes human peripheral blood T cell CD3 antigen.

Reactivity: Human, others not tested

Host / Isotype:Mouse, IgG2a Kappa

Formulation:Lyophilized from a solution in 0.01M PBS pH7.2

Reconstitution:Double distillated water is recommended to adjust the final concentration to 1.00mg/mL.

Storage: Store at -20^oC

Research Area:Immunology and Oncology

Background:

CD3 is a surface marker expressed on the cell membrane of all matured T-cells and is virtually not found on the surface of other cells. Therefore, CD3 is used as a T-cell specific marker. In addition, CD3 is involved with T-cell activation by functioning as a co-receptor in the T-cell receptor complex (which is comprised of T cell receptor, ζ-chain and CD3).

Applications:Immuno-fluorescence Staining

References:

1. Kebing Wang et al., Dendritic cells transduced with a PSMA-encoding adenovirus and cocultured with autologous cytokine-induced lymphocytes induce a specific and strong immune response against prostate cancer cells. Urologic Oncology: Seminars and Original Investigations 27 (2009) 26–32.

2. Jun Pang et al., Enhanced antitumor effects by the coculture of allotumor RNA-pulsed dendritic cells with autologous cytokine-induced killer cells on hormone-refractory prostate cancer. Cancer Investigation, 25:527–534, 2007.

3. Jian Wang et al., Inducible Costimulator (ICOS) Enhances the Cytolytic Activity of Cytokine-Induced Killer Cells Against Gallbladder Cancer In Vitro and In Vivo. Cancer Investigation, 27:244–250, 2009.

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.