Description:Mouse monoclonal antibody against serine133 phosphorylated human cAMP response element-binding protein (p-S133-CREB)
Purification:Protein G affinity purified
Product Type:Primary antibody
Target Protein:Serine133 phosphorylated human cAMP response element-binding protein (p-S133-CREB)
Immunogen:Serine133 phosphorylated human CREB peptide fragment conjugated with KLH
Fusion Myeloma:Sp2/0-Ag14
Specificity:Reactive to serine133 phosphorylated human CREB peptide fragment, and reactive to KLH or BSA conjugated serine133 phosphorylated human CREB peptide fragment. Not reactive to un-conjugated KLH or BSA.
Species Reacitvity:Human, others not tested
Host / Isotype: Mouse, IgG2 Kappa
Formulation:Lyophilized form from a solution of 0.01M PBS, pH7.0
Reconstitution: Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.
Storage:Store at -20oC. Avoid freeze and thaw cycles.
Research Area:Signal transduction, gene transcription
Background:
cAMP response element-binding protein (CREB) is a transcription factor that regulates the transcription of a wide spectrum of genes in response to second massagers (cAMP and Ca++) and cell growth or stress signals. The primary binding sites ofCREBon the promoter regions are cAMP response element (CRE) and calcium response element which have a common 5’-TGACGTCA-3’ motif.CREBcan be phosphorylated and activated by cAMP-dependent protein kinase A, calcium/calmodulin-dependent kinase, and kinases in the mitogen-activated kinase (MAPK) and extracelluar signal-regulated kinase (ERK) signal cascades.
CREB plays an important role in neuroplasticity and memory formation by controlling the expression of c-fos, brain-derived neurotrophic factor, tyrosine hydrolase and many neuropeptides.
Application:
In indirect ELISA, the antibody is reactive to serine133 phosphorylated humanCREBpeptide and reactive to serine133 phosphorylated humanCREBpeptide conjugated with KLH or BSA. The peptide and the conjugates were coated at 1μg/ml in pH 9.5 carbonate buffer.
Other applications have not been evaluated.
References:
If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.
Product Specificity | mAb anti-Human Phospho-CREB [p-S133], B1C4 |
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Application | EIA |
Size | 0.1 mg |
Anogen-Yes Biotech Laboratories Ltd.是一家位于加拿大密西沙加的生物技术公司。成立于1989年,我们30年来一直是高质量抗体基产品的骄傲供应商。我们提供300多种单克隆和多克隆抗体,以及60多种类型的ELISA试剂盒,用于研究,诊断和治疗应用。
作为一家专注于研究的公司,我们还开发了许多专有的诊断和治疗产品。我们的产品Abcream是一种有效的抗牛皮癣局部抗IL-8(CXCL8)单克隆抗体,已于2001年获得中国国家食品药品监督管理局(SFDA)的批准,并获得多项国际专利。我们的用于诊断目的的新生儿TSH ELISA试剂盒也已获得加拿大卫生部的许可。我们还启动了针对炎症,阿尔茨海默氏病和癌症的研究和开发产品。