INTENDED USE

Pre-titrated Reagent Kit for human MCP-3 contains calibrated MCP-3 standards and pre-titrated capture and detection antibodies for quantitative measurement of human MCP-3 in biological samples by enzyme-linked immunoassay (ELISA). An assay protocol is provided with the kit to use the reagents in 96-well ELISA format. It is recommended to dilute all reagents according to this manual. The lyophilized standards have been calibrated against WHO reference material to ensure accuracy and consistence of test results. The reagents can also be used with 384-well ELISA plate and optimized for use in other type of immunoassays.

Background

Macrophage/monocyte chemotactic protein-3 (MCP-3), like other two closely related chemokines (MCP1 and MCP-2), is chemotactic to monocytes, but not to neutrophils. MCP-3 showed 78% sequence homology with MCP-1 and 58% sequence homology with MCP-2. The primary receptor for MCP-3 is D6. MCP-3 also competes with MCP-1 to bind to CCR10, and competes with Rantes and MIP-1alpha to bind to CCR1. MCP-3 stimulates macrophage to secrete proteases. A variety of cancer cell lines express MCP-3, suggesting a role in tumor invasion and metastasis.