RACTIVITY | Human |
SENSITIVITY | <4 pg/mL |
ASSAY RANGE | 4-1000 pg/mL |
REAGENTS PROVIDED | IL-10 MICROTITER PLATEBIOTIN CONJUGATEAVIDIN CONJUGATEIL-10 STANDARDCALIBRATOR DILUENT ICALIBRATOR DILUENT II(5X)WASH BUFFER (20X/96 wells, 30X/192 wells)SUBSTRATE ASUBSTRATE BSTOP SOLUTION |
INTENDED USE
This Human Interleukin 10 ELISA Kit is to be used for the in vitro quantitative determination of human interleukin 10 (IL-10) concentrations in cell culture supernatant, serum, plasma, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not to be used in diagnostic or therapeutic procedures.
INTRODUCTION
The mature human IL-10 is a protein with 160 amino acids and the functional IL-10 is a homodimer. IL-10 is expressed by a variety of cells, including mouse T cells (TH1, TH2, and Tr1 subsets), B cells derived from peripheral blood, tonsils or spleen, Epstein–Barr virus (EBV)-transformed B cell lines, Burkitt’s lymphoma, AIDS lymphomas, monocytes, placental trophoblasts, bronchial epithelial cells, and certain tumor cells including melanomas and carcinomas of various origin.
IL-10 plays an important role in inflammatory and immune responses. The biological activities of IL-10 include both immunosuppressive and immuno-stimulatory effects. For example, IL-10 can suppress the production of pro-inflammatory cytokines by monocytes and neutrophils, and down regulates the expression of activating and co-stimulatory molecules on monocytes and dendritic cells. IL-10 also can improve the growth of B cells and mast cells, and inhibit or enhance the activities of T cells depending on their activation conditions. The different polymorphisms in the IL-10 gene promoter have been demonstrated the association with both SIDS (sudden infant death syndrome) and sudden unexpected death due to infection.
Based on the functions of IL-10, it has been suggested that an IL-10 antagonist can be applied to boost anti-viral immunity against viruses such as EBV and that the IL-10 molecule itself may be used as an anti-inflammatory reagent.
This IL-10 ELISA is a ready-to-use 3.5-hour solid phase immunoassay capable of measuring IL-10 levels in cell culture supernatant, serum, plasma, and other biological fluids in the range of 0 to 1600 pg/mL. This assay has shown no cross-reactivity with other cytokines tested.
Product Specificity | Human IL-10 ELISA Kit |
---|---|
Application | Refer to Insert |
Size | 96 wells |
Anogen-Yes Biotech Laboratories Ltd.是一家位于加拿大密西沙加的生物技术公司。成立于1989年,我们30年来一直是高质量抗体基产品的骄傲供应商。我们提供300多种单克隆和多克隆抗体,以及60多种类型的ELISA试剂盒,用于研究,诊断和治疗应用。
作为一家专注于研究的公司,我们还开发了许多专有的诊断和治疗产品。我们的产品Abcream是一种有效的抗牛皮癣局部抗IL-8(CXCL8)单克隆抗体,已于2001年获得中国国家食品药品监督管理局(SFDA)的批准,并获得多项国际专利。我们的用于诊断目的的新生儿TSH ELISA试剂盒也已获得加拿大卫生部的许可。我们还启动了针对炎症,阿尔茨海默氏病和癌症的研究和开发产品。