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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
yesbiotech/HAMA ELISA Kit/96 wells/EL10036
产品编号:EL10036
市  场 价:¥8100.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$405.00
品      牌: yesbiotech
公      司:yesbiotech
公司分类:
yesbiotech/HAMA ELISA Kit/96 wells/EL10036
商品介绍
  • Description
  • Additional

Description

Details

RACTIVITY

Human

SENSITIVITY

<3 ng/mL

ASSAY RANGE

3-400 ng/mL

REAGENTS PROVIDED

HAMAMICROTITER PLATEHRP CONJUGATEHAMA STANDARDCALIBRATOR DILUENTWASH BUFFER (20X)SUBSTRATE ASUBSTRATE BSTOP SOLUTION

INTENDED USE

This HAMA ELISA is to be used for the quantitative determination of human anti-mouse antibody (HAMA) in serum. It is useful for the prognosis, monitoring and follow-up patients treated with mouse IgG. This kit is intended for LABORTAROY RESEARCH USE ONLY and is not for use in diagnostic or therapeutic procedures.

INTRODUCTION

Human Anti-Mouse Antibody (HAMA) are antibodies found in human serum which have the ability to bind to mouse immunoglobulin G (IgG). The presence of HAMA is the result of an immune reaction following an exposure to mice or other similar agent, which was able to induce the immune system to generate an antibody able to bind to mouse IgG. HAMA is commonly found in patients following treated with mouse monoclonal antibodies associated with some therapeutic or diagnostic procedures. Some human auto-antibodies, the most common of which is rheumatoid factor (Rf), by virtue of their cross reactivity are able to bind mouse IgG. The HAMA assay measures HAMA across a wide dynamic range and it is not necessary to predilute human serum samples before the HAMA ELISA testing.

PRINCIPLE OF THE ASSAY

This assay applies an enzyme-linked immunosorbent assay (ELISA) technique. A purified mouse IgG has been pre-coated onto the wells of microplate. Standard (rabbit anti-mouse IgG) and specimens are added into the appropriate wells simultaneously with a HRP-labelled mouse IgG, and incubated. The Human Anti-Mouse Antibody (HAMA), if present, will bind with the mouse IgG which coated on the wells and HRP-labelled mouse IgG as well, forming a "Sandwich". Following a wash to remove any unbound substance, a substrate solution is added. The developed colour is directly proportional to the anti-mouse antibody concentration in the specimen and Standard samples.

This ELISA Kit includes a Calibration Diluent for preparing a 2-fold dilution of Standard series. It allows the operator to produce a standard curve with Optical Density (O.D) versus Human Anti-Mouse Antibody concentration (ng/mL). The concentration of Human Anti-Mouse Antibody in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Additional

Additional Information

Product SpecificityHAMA ELISA Kit
ApplicationRefer to Insert
Size96 wells
品牌介绍

Anogen-Yes Biotech Laboratories Ltd.是一家位于加拿大密西沙加的生物技术公司。成立于1989年,我们30年来一直是高质量抗体基产品的骄傲供应商。我们提供300多种单克隆和多克隆抗体,以及60多种类型的ELISA试剂盒,用于研究,诊断和治疗应用。


作为一家专注于研究的公司,我们还开发了许多专有的诊断和治疗产品。我们的产品Abcream是一种有效的抗牛皮癣局部抗IL-8(CXCL8)单克隆抗体,已于2001年获得中国国家食品药品监督管理局(SFDA)的批准,并获得多项国际专利。我们的用于诊断目的的新生儿TSH ELISA试剂盒也已获得加拿大卫生部的许可。我们还启动了针对炎症,阿尔茨海默氏病和癌症的研究和开发产品。


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