Description:Mouse anti-Human Thyroid Stimulating Hormone (TSH) monoclonal antibody

Purification:Protein G affinity purified

Product Type: Primary antibody-capture antibody in matched antibody pair

Target Protein:Human TSH

Immunogen:Recombinant human TSH

Fusion Myeloma:Not known

Specificity:Human TSH αsubunit

Species Reacitvity: Human, others not tested

Host / Isotype: Mouse, IgG1 Kappa

Formulation:Lyophilized from a solution in 0.01M PBS, pH 7.2

Reconstitution:Double distillated water is recommended to adjust the final concentration to 1.00 mg/mL.

Storage: Store at -20^oC

Research Area:Hormone, metabolism

Background:

Thyroid stimulating hormone is secreted by pituitary gland in response to the stimulation of Thyrotropin Releasing Hormone (TRH) produced by hypothalamus. TSH binds to the TSH receptors on the surface of the follicular cells in thyroid and stimulates the production of thyroid hormones (T4 and T3). TSH, T3 and T4 play an important role in normal growth, brain development in young children, and regulate the metabolism in adults. TSH secretion is increased when T3 and T4 are insufficient. Elevated TSH, but low T3 and T4 levels indicate that thyroid cannot produce thyroid hormones at a normal rate and the condition is called hypothyroidism. If T3 and T4 levels are high when TSH is at a low level, it indicates that thyroid is producing abnormally large amount thyroid hormones, and the condition is called hyperthyroidism which is manifested by hypermetabolic symptoms.

TSH has an αsubunit that is identical to the one in other glycoprotein hormones such as LH, FSH and HCG.

Application:

ELISA:This antibody can be used as capture antibody in sandwich ELISA for human TSH detection in combination with HRP conjugated TSHb antibody [Cat No: MO-M40046T] that is derived from anti-TSH clone TSH2 [Cat No: MO-M40046A]. The suggested coating concentration is 4μg/mL.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.