Description:Mouse monoclonal antibody to human serum amyloid A (SAA)

Purification:Protein G affinity purified

Product Type:Primary antibody- capture antibody in matched antibody pair

Target Protein:Human SAA

Immunogen:Highly purified recombinant human SAA (MW: 12 kDa)

Fusion Myeloma:Sp2/0-Ag14

Specificity:This monoclonal antibody reacts with natural and recombinant human SAA.

Species Reacitvity: Human, others not tested

Cross-Reactivity: Does not show any cross-reaction with other human cytokines or growth factors tested such as IL-1β, IL-8, MCAF, TGF-β and EGF.

Host / Isotype: Mouse, IgG1 Kappa

Formulation:Lyophilized from 0.01M K₂HPO₄– KH₂PO₄buffer, pH = 7.0

Reconstitution:Double distilled water is recommended to reconstitute the antibody.

Storage: Store at -20^oC

Research Area:Acute phase protein. Inflammatory reaction.

Background:

SAA is an acute phase protein and a sensitive inflammatory marker. Cytokines such as IL-1, IL-6 and TNF-alpha stimulates hepatocytes to produce and release SAA. In acute phase, SAA in blood can increase to more than 1,000 folds of normal level. Prolonged and repeated elevation of SAA results in abnormal deposition of amyloid proteins in organ or tissue in insoluble beta pleated form. Determination of SAA levels is important for assessment of renal allograft rejection, estimation of tissue damage caused by myocardial infarction and severe angina, and diagnosis of inflammation in cystic fibrosis patients and in patients infected with virus.

Application:

ELISA: This antibody can be used as a capture antibody in sandwich ELISA applications for human SAA detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated, Cat. No.: MO-C40028T; or Biotin-conjugated, Cat. No.: MO-40028TB). Suggested capture coating dose: 0.3 μg/well.

Western Blot: The antibody, when used at concentration of 0.1-0.5 μg/mL will allow visualization of 100ng/lane of recombinant human SAA.

References:

1. Wu TL, I Chen Tsai, Chang PY, Tsao KC, Sun CF, Wu LL, Wu JT.Establishment of an in-house ELISA and the reference range for serum amyloid A (SAA): complementarity between SAA and C-reactive protein as markers of inflammation. Clin Chim Acta. 2007 Feb; 376(1-2):72-6.

2. Chandrabala Shah et al. Serum amyloid A is an innate immune opsonin for Gram-negative bacteria. Blood. 2006 108: 1751-1757.

3. Chang Yeop Han et al. Differential Effect of Saturated and Unsaturated Free Fatty Acids on the Generation of Monocyte Adhesion and Chemotactic Factors by Adipocytes. Diabetes. Feb 2010; 59(2): 386-396.

4. Dobrin Nedelkov et al. Investigating diversity in human plasma proteins. Proc. Natl. Acad. Sci. U.S.A. Aug 2, 2005; 102(31): 10852-10857.

5. Fan CY, Hou YR, Adak AK, Waniwan JT, dela Rosa MAC, Low PY, Angata T, Hwang KC, Chen YJ, Lin CC.Boronate affinity-based photoactivatable magnetic nanoparticles for the oriented and irreversible conjugation of Fc-fused lectins and antibodies. Chem Sci 2019; 37(10):8600-8609.