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℡ 4000-520-616
℡ 4000-520-616
yesbiotech/Human Chorionic Gonadotropin (hCG) ELISA Kit/96 wells/EL10031
产品编号:EL10031
市  场 价:¥5400.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$270.00
品      牌: yesbiotech
公      司:yesbiotech
公司分类:
yesbiotech/Human Chorionic Gonadotropin (hCG) ELISA Kit/96 wells/EL10031
商品介绍
  • Description
  • Additional

Description

Details

RACTIVITY

Human

SENSITIVITY

<3.0 mIU/mL

ASSAY RANGE

3-240 mIU/mL

REAGENTS PROVIDED

hCG MICROTITER PLATECONJUGATEhCG STANDARD - 240 mIU/mLhCG STANDARD - 120 mIU/mLhCG STANDARD - 60 mIU/mLhCG STANDARD - 30 mIU/mLhCG STANDARD - 7.5 mIU/mLhCG STANDARD - 0 mIU/mLSUBSTRATE ASUBSTRATE BSTOP SOLUTIONSAMPLE DILUENT

INTENDED USE

This hCG ELISA Kit is to be used for the in vitro quantitative determination of intact human chorionic gonadotropin (hCG) concentrations in serum. This kit is intended FOR LABORATORY RESEARCH USE ONLY and is not to be used for diagnostic or therapeutic procedures.

INTRODUCTION

Human chorionic gonadotropin is a glycoprotein hormone produced by normal trophoblast cells of the placenta during pregnancy. It is also is produced by trophoblast cells in hydatidiform mole and choriocarcinoma (trophoblast diseases), and in patients with germ cell tumors (testicular choriocarcinoma, placental site tumors and germ cell carcinomas of the ovary) and sometimes in those with other malignancies. Small amount of hCG may also be produced by the pituitary gland. Human CG (hCG) is the hormone associated with the maintenance of pregnancy.

Human chorionic gonadotropin is a member of the glycoprotein hormone (GPH) family. Like the other glycoprotein hormones, follicle stimulating hormone (FSH), luteinizing hormone (LH) and thyroid stimulating hormone (TSH), hCG is composed of two subunits, alpha and beta. Alpha subunits of these various glycoprotein hormones are structurally very similar, but beta subunits differ in amino acid sequences. These differences are responsible for their biological and immunological specificity. The alpha-subunit of hCG is a glycopeptide of 92 amino acids and the ß-subunit of hCG is a glycopeptide of 145 amino acids. HCG has a molecular weight of 50,000 daltons, consists of an alpha subunit of 18,000 daltons and a beta subunit of 32,000 daltons. Recent elucidation of the crystal structure of hCG has revealed that all these subunits share the so-called cystin-knot structural motif with growth factors such as nerve (NGF), platelet-derived (PDGF). Although the pituitary secretes three related glycoprotein hormones, LH, FSH, and TSH, hCG is the only one to produced by the placenta in primates to maintain the steroid hormone secretions of the corpus luteum.

Predominantly intact hCG is present in serum and urine samples in normal pregnancy, as well as in hydatidiform mole. In women with extrauterine or ectopic pregnancies, unduly low hCG levels may be detected. Variable levels of hCG mostly intact hCG, may be present from individuals with persistent trophoblastic disease. Elevated concentrations are also present in cases of early pregnancy loss (EPL) or biochemical pregnancy, gestational Down syndrome. Bladder cancer, ovarian cancer and certain other malignancies may generate a small amount of hCG alpha and beta subunit. Commonly, the amount of subunit is insufficient for combination to occur to make intact hCG.

PRINCIPLE OF THE ASSAY

This hCG enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to hCG. Standards or samples are added to the microtiter plate wells and incubated. The plate wells are washed to remove any unbound standard or sample, hCG, if present, will bind to the antibody pre-coated on the wells. A standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific to hCG is added to each well to “sandwich” the hCG immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3", 5,5" Tetramethyl-benzidene) substrate solution is added to each well. This enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain hCG and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

In order to measure the concentration of hCG in the sample, this ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus hCG concentration (mIU/mL). The concentration of hCG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

CITATIONS

1. van den Heuvel MJ, Hatta K, Peralta CG, Han VK, Clark DA. CD56+ cells are recruited to the uterus in two waves: at ovulation and during the first 2 weeks after missed menses. Am J Reprod Immunol. 2008 Feb;59(2):90-8. Epub 2007 Nov 21.

Additional

Additional Information

Product SpecificityHuman Chorionic Gonadotropin (hCG) ELISA Kit
ApplicationRefer to Insert
Size96 wells
品牌介绍

Anogen-Yes Biotech Laboratories Ltd.是一家位于加拿大密西沙加的生物技术公司。成立于1989年,我们30年来一直是高质量抗体基产品的骄傲供应商。我们提供300多种单克隆和多克隆抗体,以及60多种类型的ELISA试剂盒,用于研究,诊断和治疗应用。


作为一家专注于研究的公司,我们还开发了许多专有的诊断和治疗产品。我们的产品Abcream是一种有效的抗牛皮癣局部抗IL-8(CXCL8)单克隆抗体,已于2001年获得中国国家食品药品监督管理局(SFDA)的批准,并获得多项国际专利。我们的用于诊断目的的新生儿TSH ELISA试剂盒也已获得加拿大卫生部的许可。我们还启动了针对炎症,阿尔茨海默氏病和癌症的研究和开发产品。


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