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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
yesbiotech/High Sensitivity Human Growth Hormone (H-hGH) ELISA Kit/96 wells/EL10030H
产品编号:EL10030H
市  场 价:¥5400.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$270.00
品      牌: yesbiotech
公      司:yesbiotech
公司分类:
yesbiotech/High Sensitivity Human Growth Hormone (H-hGH) ELISA Kit/96 wells/EL10030H
商品介绍
  • Description
  • Additional

Description

Details

RACTIVITY

Human

SENSITIVITY

<0.1 ng/mL

ASSAY RANGE

0.1-12 ng/mL

REAGENTS PROVIDED

MICROTITER PLATECONJUGATEhGH STANDARD – 12 ng/mLhGHSTANDARD – 6 ng/mLhGHSTANDARD – 2 ng/mLhGHSTANDARD – 0.8 ng/mLhGHSTANDARD – 0.2ng/mLhGHSTANDARD – 0 ng/mLSAMPLE DILUENTSUBSTRATE ASUBSTRATE BSTOP SOLUTIONWASH BUFFER (20X)

INTENDED USE

This high-sensitivity Human Growth Hormone ELISA Kit is to be used for the in vitro quantitative determination of human growth hormone (hGH) concentrations in serum. This kit is intended FOR LABORATORY RESEARCH USE ONLY, and is not to be used in diagnostic or therapeutic procedures.

INTRODUCTION

Human growth hormone (GH) is a 22kDa monomeric protein produced and stored in somatotrophs in the anterior pituitary gland. GH is released from the pituitary into the bloodstream in a pulsatile manner under the regulatory control of hypothalamic somatostatin (SS) and GH-releasing factor (GHRF) [1]. The timing and frequency of GH release appears to be regulated by somatostatin, while the amplitude of GH release is determined by GHRF. A minor fraction (~10%) of GH in circulation exists in a smaller 20 kDa form [2]. GH has profound effects on tissue growth and metabolism, which is thought to be mediated through GH-dependent production of IGF-I and IGF-II, and their associated binding proteins. GH apparently stimulates IGF production after binding to specific cell surface receptors in the liver and, possibly, other tissues. Almost 50% of GH in circulation is bound to a high affinity GH binding protein (GHBP), which represents the extracellular domain of the GH receptor. Deficient GH secretion can occur in a number of clinical conditions [3]. However, evaluation of GH deficiency is complicated by the episodic nature of GH secretion and low circulating levels. A variety of physiologic and pharmacologic stimuli have been used to stimulate pituitary GH release during testing and failure to achieve a normal serum GH level in response to at least 2 GH stimulation or provocative tests is considered to be a diagnostic of GH deficiency [4]. The definition of a normal serum GH response is controversial, although published values generally range from 5 to 10 ng/mL. GH excess (or acromegaly) can be caused either by direct GH hypersecretion or GH excess secondary to GHRF hypersecretion.

PRINCIPLE OF THE ASSAY

This hGH enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to hGH. Standards or samples are then added to the microtiter plate wells and incubated. After wash all wells, hGH if present, will bind to the antibody pre-coated on the wells. In order to quantitatively determine the amount of hGH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific to hGH is added to each well to “sandwich” the hGH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, a TMB (3,3", 5,5" Tetramethyl-benzidene) substrate solution is added to each well. This enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain hGH and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

In order to measure the concentration of hGH in the sample; this Human hGH ELISA Kit includes a set of calibration standards (6 standards). The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density (O.D.) versus GH concentration (ng/mL). The concentration of hGH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

CITATIONS

1. Rempel M, Yardley JE, MacIntosh A, Hay JL, Bouchard D, Cornish S, Marks SD, Hai Y, Gordon JW, McGavock J.Vigorous Intervals and Hypoglycemia in Type 1 Diabetes: A Randomized Cross Over Trial. Sci Rep 2018; 1(8):15879.

Additional

Additional Information

Product SpecificityHigh Sensitivity Human Growth Hormone (H-hGH) ELISA Kit
ApplicationRefer to Insert
Size96 wells
品牌介绍

Anogen-Yes Biotech Laboratories Ltd.是一家位于加拿大密西沙加的生物技术公司。成立于1989年,我们30年来一直是高质量抗体基产品的骄傲供应商。我们提供300多种单克隆和多克隆抗体,以及60多种类型的ELISA试剂盒,用于研究,诊断和治疗应用。


作为一家专注于研究的公司,我们还开发了许多专有的诊断和治疗产品。我们的产品Abcream是一种有效的抗牛皮癣局部抗IL-8(CXCL8)单克隆抗体,已于2001年获得中国国家食品药品监督管理局(SFDA)的批准,并获得多项国际专利。我们的用于诊断目的的新生儿TSH ELISA试剂盒也已获得加拿大卫生部的许可。我们还启动了针对炎症,阿尔茨海默氏病和癌症的研究和开发产品。


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