Description:Mouse monoclonal antibody to human hepatitis C virus (HCV) non-structural protein NS-4

Purification:Protein G affinity purified

Product Type:Primary Antibody

Target Protein:Human hepatitis C virus (HCV) non-structural protein NS-4

Immunogen:Purified recombinant chimeric HCV polyprotein (555 amino acid residues)

Fusion Myeloma:Sp2/0-Ag14

Specificity:The mAb reacts with synthetic NS-4a protein (residues 1689-1735 on HCV polyprotein) and recombinant chimeric HCV polyprotein (60 kDa).

Species Reactivity:Human hepatitis C virus, others not tested

Cross-Reactivity:No cross reaction was seen with recombinant core protein C + envelope protein M (residues 1-142), synthetic core protein C (residues 1 -61), recombinant NS-3 protein (residues 1252-1477) and synthetic NS-3 protein (residues 1378-1458).

Host / Isotype:Mouse, IgG1 Kappa

Formulation:Lyophilizedfrom a solution in 0.01M PBS, pH 7.0

Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL

Storage: Store at -20^oC

Research Area:Virology

Background:

Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of core protein (capsid protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.

Application:

ELISA:Reacts with HCV NS-4.

Western Blot: The mAb, when used at concentration of 0.5μg/ml, will allow visualization of 0.1μg/lane of synthetic NS-4 peptide and 0.1μg/lane recombinant chimeric HCV polyprotein

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.