Description:Biotin labeled mouse monoclonal antibody (mAb) to human PD-1, or biotin labeled mouse mAb to human CD279.

Purification:Protein G affinity purified

Product Type:Detection antibody in matched antibody pair, biotin conjugated

Target Protein:Human programmed cell death-1 (PD-1)

Immunogen:Human cell expressed recombinant programmed cell death-1 (Pro21-Gln162 with a histidine tag at C-terminus). Accession No.: NP-005009.2

Fusion Myeloma:Sp2/0-Ag14

Specificity:The mAb is reactive to human cell expressed programmed cell death-1 (PD-1).

Species Reactivity:Human, others not tested.

Host / Isotype:Mouse, IgG1 Kappa

Formulation:0.01M PBS, pH 7.2 containing 0.5% gelatin

Storage: Store at -20^oC. Avoid freeze and thaw cycles

Research Area:Immunosuppression

Background:

Programmed Cell Death-1 (PD-1), also called CD279, is a 288 amino acid cell surface protein that belongs to the immunoglobulin superfamily. The protein is expressed on T cells, B cells and macrophages. When PD-1 is joined with its ligand (CD274 or B7-H1), the immune cell’s activation and effector function are attenuated. Research suggested that PD1 pathway is mainly involved with attenuation at periphery sites while the B7/CTLA-4 pathway is primarily involved with the attenuation of the activation of immune cells in secondary lymphoid organs. PD-1 pathway is implicated with cancer’s immune-evasion, autoimmunity, and Listeria monocytogenes infection.

Application:

Sandwich ELISA:Biotin conjugated anti PD-1 clone 7G12, when used in combination with clone 7C9 (MO-L4003B) coated ELISA plate, can detect human cell expressed PD-1 in Sandwich ELISA application.

In addition to the immunogen, the antibody also reacts with another human cell expressed recombinant PD-1 (Leu25-Gln167 with an Fc tag at C-terminus). Accession No.: Q15116.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.