Description:Mouse monoclonal antibody to human hepatitis C virus (HCV) non-structural protein NS-4

Purification:Protein G affinity purified

Product Type:Primary Antibody

Target Protein:Human hepatitis C virus (HCV) non-structural protein NS-4

Immunogen:A synthetic polypeptide of at least 90 residues in length, selected from the immunodominant NS-4 region of Chinese HCV strains

Fusion Myeloma:Sp2/0-Ag14

Specificity:The mAb reacts with synthetic NS-4a protein, and with recombinant chimeric HCV polyprotein (60 kDa).

Species Reactivity:Human hepatitis C virus, others not tested

Cross-Reactivity:No cross-reaction with HCV core region and other non-structural region.

Host / Isotype:Mouse, IgG1 Kappa

Formulation:Lyophilized from a solution in 0.01M PBS, pH 7.0

Reconstitution:Double distilled water is recommended to adjust the final concentration to 1.00mg/mL

Storage: Store at -20^oC

Research Area:Virology

Background:

Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of core protein (capsid protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.

Application:

ELISA:Reacts with HCV NS-4.

Western Blot:The mAb, when used at concentration of 0.5μg/ml, will allow visualization of 0.5μg/lane of synthetic NS-4 peptide and 0.1μg/lane recombinant chimeric HCV polyprotein.

Immunohistochemistry: The antibody has been used in immunoperoxidase-avidin-biotin (ABC) assay for Formalin-fixed paraffin embedded tissue section.

References:

If research is published using this product, please inform Anogen in order to cite the reference on this datasheet. Anogen will provide one unit of product in the same category as gratitude.